collagen 2  (Developmental Studies Hybridoma Bank)

Journal: JBMR Plus

Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

doi: 10.1093/jbmrpl/ziag021

Figure Lengend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

Techniques: Immunohistochemical staining, Staining

Journal: JBMR Plus

Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

doi: 10.1093/jbmrpl/ziag021

Figure Lengend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

Techniques: Immunohistochemical staining, Staining

Journal: eLife

Article Title: Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of osteoarthritis

doi: 10.7554/eLife.103332

Figure Lengend Snippet: Representative images of a rat with DMM surgery ( A ) with MSC injection (TdTomato – B) and collagen 2 staining ( C ). Scale bars equal 20 µm.

Article Snippet: Antigen retrieval was achieved using 10 mM sodium citrate (pH 6.0), and non-specific blocking was prevented using goat serum (1:500 dilution in TBST). tdTomato (AB8181, Origene), Collagen 2 (Col2; Clone # II-II6B3, DSHB), PRG4 (Clone # 9G3, Millipore), Ccl2 (Clone # 2D8, Thermo Fisher), Ki67 (Clone # SolA15, ThermoFisher), Sox9 (Clone # 7H13L8, Thermo Fisher) or CD47 (Clone # B6H12, Thermo Fisher) were applied to the sections and incubated overnight.

Techniques: Injection, Staining

Journal: eLife

Article Title: Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of osteoarthritis

doi: 10.7554/eLife.103332

Figure Lengend Snippet: The gating strategy to identify and sort CD47 Hi vs. CD47 Lo cell populations ( A–C ). CD47 Hi vs. CD47 Lo cells were identified and isolated from normal and osteoarthritis (OA) synovial tissue ( B ). Chondrogenic differentiation of CD47 Hi vs. CD47 Lo cells using pellet culture and stained with Alcian Blue ( D ). GAG quantification of the pellets ( E ). The pellets were also stained with Collagen 2 (Col2) as a marker of mature cartilage ECM ( E, G ). Signifiance determined by1 way ANOVA. p <0.05. Scale bars equal 100 µm.

Article Snippet: Antigen retrieval was achieved using 10 mM sodium citrate (pH 6.0), and non-specific blocking was prevented using goat serum (1:500 dilution in TBST). tdTomato (AB8181, Origene), Collagen 2 (Col2; Clone # II-II6B3, DSHB), PRG4 (Clone # 9G3, Millipore), Ccl2 (Clone # 2D8, Thermo Fisher), Ki67 (Clone # SolA15, ThermoFisher), Sox9 (Clone # 7H13L8, Thermo Fisher) or CD47 (Clone # B6H12, Thermo Fisher) were applied to the sections and incubated overnight.

Techniques: Isolation, Staining, Marker

Journal: Journal of Pharmaceutical Analysis

Article Title: Signatures of proteomics and glycoproteomics revealed liraglutide ameliorates MASLD by regulating specific metabolic homeostasis in mice

doi: 10.1016/j.jpha.2025.101273

Figure Lengend Snippet: Identification and validation of potential signature molecules affected by liraglutide (Lira). (A) Protein-protein interaction (PPI) analysis of differential proteins and glycoproteins in major pathways. Node size reflects degree value, with red representing glycoproteins and blue representing proteins. Color-coded segments show pathway associations. (B) Messenger RNA (mRNA) expression of Acaa2 , Lamc1 , and Col4a2 (for proteins), and Acox1 , Gclc , and Shmt2 (for glycoproteins). (C) Protein expression of laminin subunit gamma-1 (LAMC1), collagen type IV alpha-2 chain (COL4A2), and acetyl-CoA acyltransferase 2 (ACAA2) (for proteins), and glutamate-cysteine ligase catalytic subunit (GCLC) (for glycoproteins). Data is presented as mean ± standard deviation (SD) ( n = 3 per group). P values were calculated using Student's t -test or one-way analysis of variance (ANOVA) with Tukey's comparisons. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. ns: not significant. PPAR: peroxisome proliferators-activated receptor; ECM: extracellular matrix; ND: normal diet; HFD: high-fat diet; HFDL: HFD + Lira; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The primary antibodies used in our study were as follows: laminin subunit gamma-1 (LAMC1) (1:8000, 67706-1-Ig; Proteintech Group, Inc., Wuhan, China), collagen type IV alpha-2 chain (COL4A2) (1:5000, 55131-1-AP; Proteintech Group, Inc.), acetyl-CoA acyltransferase 2 (ACAA2) (1:5000, 11111-1-AP; Proteintech Group, Inc.), glutamate-cysteine ligase catalytic subunit (GCLC) (1:6000, 12601-1-AP; Proteintech Group, Inc.), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:100000, 60004-1-Ig; Proteintech Group, Inc.), and α-tubulin (1:6000, PTM-5001; PTM BIO, Hangzhou, China).

Techniques: Biomarker Discovery, Expressing, Standard Deviation